Pathogen resistant citrus compositions, organisms, systems, and methods
AssigneeThe Texas A&M University System
InventorsT. Erik MIRKOV, Javier GONZALEZ-RAMOS
Life of this patent
Estimating the term…
Abstract
The present disclosure relates, according to some embodiments, to pathogen resistant citrus compositions, organisms, systems, and methods. For example, a composition may comprise a peptide (e.g., a defensin peptide) and/or a nucleic acid (e.g., a defensin nucleic acid). A pathogen resistant citrus plant may comprise, in some embodiments, a defensin peptide and/or an expressable nucleic acid encoding a defensin peptide.
Claims31
A citrus fruit comprising at least one defensin peptide comprising a first defensin peptide, wherein the amino acid sequence of the first defensin peptide is at least about 99% identical to SEQ ID NO:
- 1, at least about 99% identical to SEQ ID NO:
- 2, at least about 99% identical to SEQ ID NO:
- 7, or at least about 99% identical to SEQ ID NO:
- 8.
A citrus fruit comprising (a) at least one spinich defensin peptide or (b) at least one spinich defensin nucleic acid or (c) at least one spinach defensin peptide and at least one spinich defensin nucleic acid.
A citrus fruit comprising at least one defensin nucleic acid comprising a first defensin nucleic acid, wherein the nucleic acid sequence of the first defensin nucleic acid is at least about 98% identical to SEQ ID NO:
- 3, at least about 98% identical to SEQ ID NO:
- 4, at least about 98% identical to SEQ ID NO:
- 5, at least about 98% identical to SEQ ID NO:
- 6, at least about 98% identical to SEQ ID NO:
- 9, at least about 98% identical to SEQ ID NO:
- 10, at least about 98% identical to SEQ ID NO:
- 11, at least about 98% identical to SEQ ID NO:
- 12, at least about 98% identical to SEQ ID NO:
- 30, or at least about 98% identical to SEQ ID NO:
- 31.
A citrus composition comprising at least one defensin peptide, wherein the amino acid sequence of the at least one defensin peptide is
- at least about 99% identical to the amino acid sequence of SEQ ID NO:
- 1,
- at least about 99% identical to the amino acid sequence of SEQ ID NO:
- 2,
- at least about 99% identical to the amino acid sequence of SEQ ID NO:
- 7, or
- at least about 99% identical to the amino acid sequence of SEQ ID NO:
- 8.
A citrus composition comprising at least one defensin nucleic acid, wherein the nucleic acid sequence of the at least one nucleic acid is
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 3,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 4,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 5,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 6,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 9,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 10,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 11,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 12,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 30, or
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 31.
A method for grafting a first citrus plant to a second citrus plant, the method comprising:
- contacting a scion of the first plant with a rootstock of the second plant,
- wherein the first plant or the second plant comprises an expression cassette, the expression cassette comprising, in a 5′ to 3′ direction:
- an expression control sequence,
- a spinach defensin nucleic acid operably linked to the expression control sequence,
- wherein the nucleic acid sequence of the spinach defensin is
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 3,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 4,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 5,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 6,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 9,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 10,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 11,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 12,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 30, or
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 31, and
- a 3′ termination sequence operably linked to the spinach defensin nucleic acid.
A method of treating a citrus plant having and/or at risk of having a microbial infection, the method comprising:
- grafting a second plant to the citrus plant having and/or at risk of having a microbial infection,
- wherein the second plant comprises an expression cassette, the expression cassette comprising, in a 5′ to 3′ direction:
- an expression control sequence,
- a spinach defensin nucleic acid operably linked to the expression control sequence,
- wherein the nucleic acid sequence of the spinach defensin is
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 3,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 4,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 5,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 6,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 9,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 10,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 11,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 12,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 30, or
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 31, and
- a 3′ termination sequence operably linked to the spinach defensin nucleic acid.
A method for enhancing a citrus plant's innate ability to respond to contact infection with a pathogen, the method comprising:
- grafting a second plant to the citrus plant,
- wherein the second plant comprises an expression cassette, the expression cassette comprising, in a 5′ to 3′ direction:
- an expression control sequence,
- a spinach defensin nucleic acid operably linked to the expression control sequence,
- wherein the nucleic acid sequence of the spinach defensin is
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 3,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 4,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 5,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 6,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 9,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 10,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 11,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 12,
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 30, or
- at least about 98% identical to the nucleic acid sequence of SEQ ID NO:
- 31, and
- a 3′ termination sequence operably linked to the spinach defensin nucleic acid.
Dependencies are inferred from the claim text.