Sample Substrate Having a Divided Sample Chamber and Method of Loading Thereof
AssigneeApplied Biosystems, LLC
InventorDonald R. SANDELL
Life of this patent
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Abstract
A sample substrate configured for samples of biological material is provided. The sample substrate has a dual chambered sample well separated by a wall that may be punctured or otherwise breached to allow mixing of material contained in the two initially separate chambers. The chambers are connected by channels to fluid reservoirs, wherein the channels can be staked to prevent further fluid flow into and out of the chambers. Methods of loading a sample substrate are also provided.
Claims15
1-17. (canceled)
A method of filling a sample substrate with a biological sample, comprising:
- filling at least a portion of a sample chamber with a first fluid through a first channel;
- filling at least a portion of a sample chamber with a second fluid through a second channel;
- and
- initiating at least one of the sample chamber portions so that the first and second fluids are in fluid communication with each other.
A sample substrate for biological samples, comprising:
- a first channel;
- a second channel distinct from the first channel;
- a sample chamber being defined by at least a first member and a second member;
- a first position in which the first member and the second member are fluidly isolated from one another, the first member is in fluid communication with the first channel, and the second member is in fluid communication with the second channel;
- and
- a second position in which the first channel is in fluid communication with the second channel;
- wherein the first member is a first wall and the second member is a second wall.
Dependencies are inferred from the claim text.