Preparation of Neutralizing Antibody to Human Herpesvirus 6 Glycoprotein Q1 and Analysis Thereof

A method of screening an inhibitor of a HHV-6B virus, the method comprising:

• A) a step of providing HHV-6B gQ1 and HHV-6B gQ2;
• B) a step of contacting a test substance with the HHV-6B gQ1 and the HHV-6B gQ2 under the condition in which the HHV-6B gQ1 and the HHV-6B gQ2 are bound;
• and
• C) a step of observing binding between the HHV-6B gQ1 and the HHV-6B gQ2, wherein when the binding is inhibited, it is determined that the test substance is an inhibitor of a HHV-6B virus.

A kit for screening an inhibitor of a HHV-6B virus, the kit comprising:

• A) HHV-6B gQ1;
• B) HHV-6B gQ2;
• and
• C) a means for providing the condition under which the HHV-6B gQ1 and the HHV-6B gQ2 are bound, wherein
• in the case where the binding is inhibited when a test substance is contacted with the HHV-6B gQ1 and the HHV-6B gQ2 under the condition in which the HHV-6B gQ1 and the HHV-6B gQ2 are bound, it is determined that the test substance is an inhibitor of a HHV-6B virus.

A method of screening a neutralizing epitope of a HHV-6B virus, the method comprising:

• A) a step of providing an antibody comprising an antigen determining region (CDR) in SEQ ID No.:
• 10 and SEQ ID No.:
• 12 or an antigen binding fragment thereof;
• B) a step of contacting a plurality of peptides being a candidate for the antibody or an antigen binding fragment thereof under the condition in which an epitope is bound;
• and
• C) a step of determining a sequence having identity or similarity in the plurality of peptides bound to the antibody or an antigen binding fragment thereof, and selecting the sequence having identity or similarity as a neutralizing epitope.

A kit for screening a neutralizing epitope of a HHV-6B virus, the kit comprising:

• A) a means for providing an antibody comprising an antigen determining region (CDR) in SEQ ID No.:
• 10 and SEQ ID No.:
• 12 or an antigen binding fragment thereof;
• B) a means for contacting a plurality of peptides being a candidate for the antibody or an antigen binding fragment thereof under the condition in which an epitope is bound;
• and
• C) a means for determining a sequence having identity or similarity in the plurality of peptides bound to the antibody or an antigen binding fragment thereof, and selecting the sequence having identity or similarity as a neutralizing epitope.