US 2014/0080121 A1 · PRIMATE T-LYMPHOTROPIC VIRUSES

Abstract

Disclosed are compositions and methods related to the isolation and identification of the primate T-lymphotropic viruses, HTLV-3 and HTLV-4. The diversity of HTLVs was investigated among central Africans reporting contact with NHP blood and body fluids through hunting, butchering, and keeping primate pets. Herein it is shown that this population is infected with a variety of HTLVs, including two retroviruses; HTLV-4 is the first member of a novel phylogenetic lineage that is distinct from all known HTLVs and STLVs; HTLV-3 falls within the genetic diversity of STLV-3, a group that has not previously been seen in humans. The present disclosure also relates to vectors and vaccines for use in humans against infection and disease. The disclosure further relates to a variety of bioassays and kits for the detection and diagnosis of infection with and diseases caused by HTLV-3 and HTLV-4 and related viruses.

Claims4

Dependent claims

A method of identifying new primate T-lymphotropic viruses comprising:

contacting a nucleic acid using a first set of primers, wherein the first set of primers is selected from the group consisting of:
(a) primers that hybridize to SEQ ID NO:
36, 53 or 81;
or
(b) SEQ ID NOs:
7 and 8, SEQ ID NOs:
9 and 10, SEQ ID NOs:
11 and 12, SEQ ID NOs:
13 and 14, SEQ ID NOs:
15 and 16, SEQ ID NOs:
17 and 18, SEQ ID NOs:
23 and 24, SEQ ID NOs:
25 and 26, SEQ ID NOs:
27 and 28, SEQ ID NOs:
29 and 30, NOs:
31 and 32, SEQ ID NOs:
33 and 34, SEQ ID SEQ ID NOs:
69 and 70, SEQ ID NOs:
71 and 72, SEQ ID NOs:
73 and 74, SEQ ID NOs:
75 and 76, SEQ ID NOs:
77 and 78, and SEQ ID NOs:
79 and 80;
amplifying the nucleic acid;
identifying any amplified nucleic acid;
and
comparing the sequence to known primate T-lymphotropic viral sequences, wherein sequence divergence greater than 5% indicates a new virus.

24 more elements

Dependencies are inferred from the claim text.

Facts

Application

14/032,914Filed 20 Sep 2013

Priority

21 Feb 2005

Published

20 Mar 2014

Term ends

Not estimatedThe source records don’t support an estimate.

Classification14 codes

C12Q 1/701
Involving virus or bacteriophage: specific hybridization probes
A61P 37/00
Drugs for immunological or allergic disorders
C07K 14/005
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from viruses
C12N 15/86
For animal cells: viral vectors

Family

Application60/654,484Us provisional application · filed 21 Feb 2005

Application11/678,596Continuation · filed 24 Feb 2007

Application12/829,125Division · filed 1 Jul 2010

Application14/032,914Division

Application12/829,125Continuation

Application11/678,596Continuation in part

US 2008/0292657 A1Published application

US 2010/0317034 A1Published application

US 7,794,998 B2Related patent

US 8,541,221 B2Related patent

US 9,435,000 B2Related patent

US 2014/0080121 A1This patent

From the US filing history. Foreign family members aren’t listed.

Primate t-lymphotropic viruses

Life of this patent

Primate t-lymphotropic viruses

Life of this patent

Abstract

Claims4