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US 2014/0072989 A1Published application
Tsh immunoassays employing scavenging reagents for cross-reacting endocrine glycoprotein hormone analogues
AssigneeAbbott Point of Care Inc.
InventorsG. Bruce Collier, John Emegbero Omakor
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Abstract
Thyroid Stimulating Hormone (TSH) immunoassays are performed using an ELISA sandwich assay that employs scavenging or sacrificial beads for reducing interference caused by cross-reacting endocrine glycoprotein hormone analogues such as Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Chorionic Gonadotropin (CG).
Claims31
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A method for performing a TSH immunoassay, the method comprising the steps of:
- (a) inserting a fluid sample into a device comprising an immunosensor in a conduit, said immunosensor having a capture antibody to TSH;
- (b) dissolving a signal antibody into said fluid sample;
- (c) amending said fluid sample with scavenger beads for one or more of follicle stimulating hormone (FSH), luteinizing hormone (LH) and chorionic gonadotropin (CG);
- (d) forming a sandwich complex on said immunosensor, said complex comprising said capture antibody, TSH, and said signal antibody;
- (e) washing uncomplexed signal antibody from the sensor with a wash fluid;
- and
- (f) detecting a signal associated with said complexed signal antibody.
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Dependencies are inferred from the claim text.