Oncovector Nucleic Acid Molecules and Methods of Use
AssigneeThe Regents of the University of California
InventorsGregory I. Frost, Mark Roman, Frank McCormick, Tanya Meyer Tamgüney
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Abstract
Provided herein are non-viral nucleic acid vectors, including non-viral oncovectors, that are autonomously replicating plasmids (ARPs). The non-viral nucleic acid vectors exhibit fusogenic activity and can exhibit other anti-tumor or cytotoxic activities. Also provided herein are methods and uses of the non-viral nucleic acid vectors for treating cancer.
Claims79
A non-viral nucleic acid vector, comprising:
- a) an origin of replication;
- b) a first open reading frame coding for a fusogenic protein;
- and
- c) a second open reading frame coding for a replication initiator protein that activates the origin of replication for episomal replication of the vector in a cell in which it is expressed.
A non-viral nucleic acid vector composition or combination, comprising:
- a) a first nucleic acid molecule comprising an origin of replication and a first open reading frame coding for a fusogenic protein;
- and
- b) a second nucleic acid molecule comprising a second open reading frame coding for a replication initiator that activates the origin of replication in the first nucleic acid molecule when the first and second nucleic acid molecule are delivered into the same host cell for episomal replication of the first nucleic acid molecule.
The non-viral nucleic acid vector of 50, wherein:
- the open reading frame coding for a prodrug modifying protein encodes a prodrug modifying protein comprising the sequence of amino acids set forth in SEQ ID NO:501 or SEQ ID NO:502 or a variant sequence thereof that exhibits at least 85% sequence identity thereto;
- or
- the open reading frame coding for a prodrug modifying protein comprises the sequence of nucleotides set forth in SEQ ID NO:
- 498 or 500 or a variant sequence thereof that exhibits at least 85% sequence identity thereto.
A non-viral nucleic acid vector, comprising:
- a) an origin of replication;
- b) a first open reading frame coding for a prodrug converting enzyme;
- and
- c) a second open reading frame coding for a replication initiator protein that activates the origin of replication for episomal replication of the vector in a cell in which it is expressed.
Dependencies are inferred from the claim text.