Isolation of lymphocytes and delivery to splenectomy patients
AssigneeChildren's Hospital of Orange County
InventorMustafa Kabeer
Life of this patent
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Abstract
The present invention includes a method and device for the isolation of lymphocytes, particularly splenic lymphocytes, for administration to patients. In particular for patients undergoing a splenectomy, administration of splenic lymphocytes may increase a patient's immunity and reduce the likelihood of post-splenectomy infections and other complications.
Claims25
A method of reducing likelihood of post-splenectomy infection in a patient, the method comprising:
- removing all or part of the patient's spleen;
- and
- harvesting lymphocytes from at least a portion of the removed spleen;
- administering the harvested lymphocytes to the patient, thereby increasing the patient's immunity and reducing the likelihood of post-splenectomy infection.
A device for the isolation of lymphocytes from tissues, wherein the device comprises:
- a top portion comprising a lid and a crank comprising a crankshaft rotatably attached to the lid and extending therethrough in a longitudinal direction, wherein the crank shaft further comprises a grinder implement attached at a distal end of the crankshaft;
- a bottom portion comprising a filtering component at the proximal end of the bottom portion;
- and
- a middle portion defining a cylindrical body, the middle portion configured to be placed between the top portion and the bottom portion, wherein the top, middle, and bottom portions are joined together to define a cavity therein, and wherein the grinder implement is configured to grind tissue placed within the cavity.
A method of obtaining a population of splenic lymphocytes, the method comprising:
- inserting all or part of a spleen tissue into a device for the isolation of lymphocytes, the device comprising a cavity configured to receive the tissue therein, the cavity comprising a grinder implement at a proximal end of the cavity and a filtering component at a distal end of the cavity;
- grinding the tissue by pressing and rotating the grinder implement against the tissue;
- supplying fluid into the cavity;
- filtering the ground tissue and fluid through the filtering component;
- and
- collecting the ground tissue and fluid in a receptacle.
Dependencies are inferred from the claim text.