Method for the production of recombinant human factor viii
AssigneeUniversidade de Sao Paulo-USP
InventorsMarcos A. A. Demasi, Mari Cleide Sogayar, Christian Colin
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Abstract
The object of the present invention is to provide methods for the production of recombinant human Factor VIII, employing specific endoproteases, thus assuring full proteolytic processing of said factor even during its biosynthesis, consequently avoiding additional purification steps. Other objects of the present invention are the recombinant human Factor VIII as obtained by said methods, pharmaceutical compositions, related uses and therapeutic methods.
Claims6
Method for the production of recombinant human factor VIII wherein comprises the introduction of coding regions for endoproteases SPC6 and/or PACE4 AI and/or PACESOL into the genome of animal cells producing Factor VIII and/or the addition of endoproteases SPC6 and/or PACE4 AI and/or PACESOL to the growing medium containing recombinant Factor VIII in a method comprising partial deletions of the portion of the coding region for factor VII corresponding to domain B and the independent production of heavy and light chains, in which paths with variable extensions, corresponding to domain B, are connected to the coding region for the light chain.
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Dependencies are inferred from the claim text.