Boone Cardiovirus
AssigneeIDEXX Laboratories, Inc.
InventorsLela Kay Riley, Judith D. Gohndrone, Matthew Howard Myles
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Abstract
The invention provides an isolated Boone cardiovirus, Boone cardiovirus polypeptides, polynucleotides and antibodies specific for Boone cardiovirus polypeptides. Also provided are methods for detection of Boone cardiovirus.
Claims21
An isolated polynucleotide molecule comprising:
- (a) SEQ ID NO:97;
- (b) a polynucleotide at least about 80%, 85%, 90%, 95%, 98% or more homologous to SEQ ID NO:97;
- (c) a polynucleotide comprising at least about 20 contiguous nucleic acids of SEQ ID NO:97;
- (d) GenBank accession number JQ864242 or JX683808;
- or (e) a complement of (a), (b), (c), or (d).
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A method of determining the presence or absence of Boone cardiovirus polynucleotides, polypeptides, or antibodies or specific binding fragments thereof that specifically bind to a Boone cardiovirus polypeptide comprising:
- (a) obtaining a test sample;
- and
- (b) determining the presence or absence of Boone Cardiovirus polynucleotides, polypeptides, or antibodies or specific binding fragments thereof in the test sample.
The method of claim 19, wherein the polypeptide/antibody complexes are detected by a technique comprising enzyme-linked immunosorbent assay (ELISA), multiplex fluorescent immunoassay (MFI or MFIA), radioimmunoassay (RIA), sandwich assay, Western blotting, immunoblotting analysis, an immunohistochemistry method, immunofluorescence assay, or a combination thereof.
A kit for detecting a Boone cardiovirus polynucleotides or polypeptides comprising at least one of:
- (a) SEQ ID NO:97;
- (b) one or more polynucleotides at least about 80%, 85%, 90%, 95%, 98% or more homologous to SEQ ID NO:97;
- (c) one or more polynucleotides comprising at least about 20 contiguous nucleic acids of SEQ ID NO:97;
- (d) one or more complements of (a), (b) or (c);
- (e) one or more substantially purified polypeptides encoded by the one or more polynucleotides of (a), (b), (c), or (d);
- (f) one or more isolated antibodies or antigen binding fragments thereof that specifically bind to the substantially purified polypeptides of (e);
- or (g) combinations thereof.
The kit of claim 21 further comprising one or more polynucleotides, one or more substantially purified polypeptides, one or more antibodies or antigen binding fragments that can detect one or more viruses, bacteria, fungi or protozoans other than Boone cardiovirus.
Dependencies are inferred from the claim text.