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US 2014/0011698 A1Published application
Simultaneous detection of biomolecules in single cells
AssigneesMorphosys AG, Albert-Ludwigs-Universität Freiburg, HSG-IMIT Institut für Mikro-und Informationstechnik
InventorsMarkus Enzelberger, Andreas Boll, Beate Diefenbach-Streiber, Guenter Roth, Felix von Stetten, Fabian Stumpf
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Abstract
The present invention provides methods, immunoassays, kits and devices pertaining to the detection of multiple biomolecules from single cells or other biological entities. It also enables the highly parallel detection of interacting biomolecules from such entities.
Claims35
A method for the detection of two or more nucleic acids, said method comprising
- (a) providing a sample comprising a cell comprising said nucleic acids,
- (b) spatially separating said cell in a compartment comprising a moiety which is able to bind derivatives of said nucleic acids,
- (c) releasing the nucleic acids from the cell,
- (d) generating derivatives of said nucleic acids,
- (e) allowing the derivatives of said nucleic acids to bind to the moiety which is able to bind the derivatives of said nucleic acids, and
- (f) detecting or identifying the derivatives of the nucleic acids,
- wherein said sample comprises at least 103 cells,
- wherein said moiety is solid-phase particle, and
- wherein in each of said cells at least two nucleic acids are detected.
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Dependencies are inferred from the claim text.