Methods for plant transformation using spectinomycin selection
AssigneeMonsanto Technology LLC
InventorsBrian Martinell, Michael W. Petersen, David A. Somers, Yuechun Wan, Edward Williams, Xudong Ye
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Abstract
The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
Claims48
A method for producing a transgenic plant containing at least two heterologous nucleic acid sequences comprising:
- (a) providing an explant comprising a first heterologous nucleic acid sequence that confers resistance to a herbicide;
- (b) transforming the explant to comprise a second heterologous nucleic acid sequence comprising a selectable marker gene conferring spectinomycin resistance;
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- (c) regenerating an explant that exhibits spectinomycin resistance into a transgenic plant containing at least two heterologous nucleic acid sequences.
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A method of producing a transgenic plant comprising:
- (a) transforming at least a first seed explant with a heterologous nucleic acid sequence comprising a selectable marker conferring tolerance to spectinomycin;
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- (b) regenerating a transgenic plant from the transformed cells, wherein the explant is contacted, prior to, concurrently with, and/or following step (a) or step (b), with at least a first media comprising spectinomycin to select transformed cells comprising said selectable marker.
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A nucleic acid construct comprising two sequences conferring resistance to spectinomycin or streptomycin, wherein the first sequence is operably linked to a promoter active in a plant cell, and the second sequence is operably linked to a promoter active in a prokaryotic cell.
Dependencies are inferred from the claim text.