Methods for using artificial polynucleotides and compositions thereof to reduce transgene silencing
AssigneeMonsanto Technology LLC
InventorStanislaw Flasinski
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Abstract
The materials and methods disclosed provide for polynucleotide molecules sufficiently divergent from polynucleotides naturally contained in plants, or polynucleotides previously introduced into plants as transgenes to permit trait stacking in plant breeding methods or plant transformation methods. The disclosure also provides for methods and compositions to detect the polynucleotides of the invention in plants.
Claims13
A method to avoid silencing of a transgene comprising SEQ ID NO:16 operably linked to a polynucleotide encoding a chloroplast transit peptide in transgenic plants comprising the steps of:
- (a) obtaining a DNA construct comprising an artificial polynucleotide molecule encoding a chloroplast transit peptide operably linked to SEQ ID NO. 35;
- (b) transforming said DNA construct into a plant cell;
- and
- (c) regenerating said plant cell into a fertile transgenic plant,
- wherein said fertile transgenic plant comprises both said artificial polynucleotide and said transgene, and wherein silencing of said artificial polynucleotide and said transgene is avoided.
3-5. (canceled)
An artificial polynucleotide molecule comprising SEQ ID NO:35.
A plant cell comprising at least two polynucleotides, wherein said two polynucleotides encode the same polypeptide and at least one of the polynucleotides is SEQ ID NO:35 operably linked to a polynucleotide encoding a chloroplast transit peptide.
12-13. (canceled)
A plant cell, seed, plant or progeny thereof comprising SEQ ID NO:35 operably linked to a polynucleotide encoding a chloroplast transit peptide.
15-27. (canceled)
Dependencies are inferred from the claim text.