Methods and Compositions for Gray Leaf Spot Resistance in Corn
AssigneeMonsanto Technology LLC
InventorsMICHAEL R. KERNS, HONGWU JIA, DAVID VINCENT BUTRUILLE, TRAVIS J. FREY, GILBERTO POZAR, KEVIN COOK, SCOTT WALKER, GREGORY HOLLAND
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Abstract
The present invention relates to the field of plant breeding. More specifically, the present invention includes a method of using haploid plants for genetic mapping of traits of interest such as disease resistance. Further, the invention includes a method for breeding corn plants containing quantitative trait loci (QTL) that are associated with resistance to Gray Leaf Spot, a fungal disease associated with Cercospora spp.
Claims23
A method of creating a population of corn plants comprising at least one allele associated with Gray Leaf Spot (GLS) resistance comprising SEQ ID NO:
- 157, 167, or 389, the method comprising the steps of:
- a) genotyping a first population of corn plants;
- b) selecting from said first population one or more corn plants containing said allele;
- and
- c) producing from said selected corn plants a second population.
A method of introgressing a Gray Leaf Spot (GLS) resistance QTL allele into a corn plant, the method comprising the steps of:
- a) crossing at least one first corn plant comprising an allele comprising SEQ ID NO:
- 157, 167, or 389 with at least one second corn plant in order to form a segregating population;
- b) screening said segregating population with one or more nucleic acid markers to determine if one or more corn plants from said segregating population contain said allele;
- and
- c) selecting from said segregating population one or more corn plants comprising said allele associated with a Gray Leaf Spot (GLS) resistance QTL.
An isolated nucleic acid molecule for detecting a molecular marker representing a polymorphism in corn DNA, wherein said nucleic acid molecule comprises at least 15 nucleotides that include or are immediately adjacent to said polymorphism, wherein said nucleic acid molecule is at least 90 percent identical to a sequence of the same number of consecutive nucleotides in either strand of DNA that include or are immediately adjacent to said polymorphism, and wherein said molecular marker is selected from the group consisting of SEQ ID NOs:
- 157, 167, and 389.
(canceled)
Dependencies are inferred from the claim text.