Soybean plant and seed corresponding to transgenic event mon87712 and methods for detection thereof

A polynucleotide probe diagnostic for the presence of event MON87712 DNA, wherein said polynucleotide probe is of sufficient length to bind to a nucleic acid molecule comprising SEQ ID NO:

• 1, SEQ ID NO:
• 2, or at least from about 51-100 consecutive nucleotides of SEQ ID NO:
• 7 or of SEQ ID NO:
• 8, or complements thereof, and wherein said polynucleotide probe hybridizes under stringent hybridization conditions with a DNA molecule comprising SEQ ID NO:
• 1, SEQ ID NO:
• 2, or at least from about 51-100 consecutive nucleotides of SEQ ID NO:
• 7 or of SEQ ID NO:
• 8, or complements thereof and does not hybridize under stringent hybridization conditions with a DNA molecule not comprising SEQ ID NO:
• 1, SEQ ID NO:
• 2, or at least from about 51-100 consecutive nucleotides of SEQ ID NO:
• 7 or of SEQ ID NO:
• 8, or complements thereof.

A method of producing a soybean plant with increased yield comprising:

• a) selfing a MON87712 comprising soybean plant, thereby producing a seed;
• b) growing said seed to produce a plurality of progeny plant;
• and
• c) selecting a progeny plant that comprises MON87712 or a progeny plant with increased yield.

A method of producing a soybean plant with increased yield comprising:

• a) crossing a MON87712 comprising soybean plant with a second soybean plant, thereby producing a seed;
• b) growing said seed to produce a plurality of progeny plants;
• and
• c) selecting a progeny plant that comprises MON87712 or a progeny plant with increased yield.

A method of determining the zygosity of a soybean plant genome comprising soybean event MON87712 DNA in a sample comprising:

• a) contacting the sample with three different primers that
• i) when used together in a nucleic acid amplification reaction with soybean event MON87712 DNA, produces a first amplicon that is diagnostic for soybean event MON87712;
• and
• ii) when used together in a nucleic acid amplification reaction with soybean genomic DNA other than MON87712 DNA, produces a second amplicon that is diagnostic for soybean wild type genomic DNA other than event MON87712 DNA;
• b) performing a nucleic acid amplification reaction;
• and
• c) detecting said first amplicon and said second amplicon;
• wherein the presence of said first and second amplicons is diagnostic of a heterozygous genome in said sample, and wherein the presence of only said first amplicon is diagnostic of a homozygous genome in said sample.