Method and apparatus for performing quantitative analysis of nucleic acid using real-time pcr
AssigneeSamsung Electronics Co., Ltd.
InventorsSoo-kwan LEE, Kyung-ho KIM, Joon-ho KIM, Sun-ok JUNG, Kak NAMKOONG
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Abstract
A method and apparatus for performing quantitative analysis of a nucleic acid by determining a curve-fitting area based on fluorescence intensity data obtained by performing PCR on a target nucleic acid; analyzing parameters for amplification efficiency and nucleic acid concentration by curve-fitting a result of performing PCR on a reference nucleic acid with a known initial nucleic acid concentration; and estimating the initial nucleic acid concentration of the target nucleic acid by performing curve-fitting on the determined curve-fitting area using the analyzed parameters.
Claims19
A method for performing quantitative analysis of a nucleic acid, the method comprising:
- determining a curve-fitting area, including a cycle at which fluorescence intensity begins to increase exponentially, based on data regarding fluorescence intensity obtained by performing PCR on a target nucleic acid;
- analyzing parameters related to amplification efficiency and nucleic acid concentration by curve-fitting a result of performing PCR on a reference nucleic acid with a known initial nucleic acid concentration;
- and
- estimating the initial nucleic acid concentration of the target nucleic acid by performing curve-fitting on the determined curve-fitting area by using the analyzed parameters.
A nucleic acid quantitative analyzing apparatus comprising:
- a curve-fitting area determining unit which determines a curve-fitting area including a cycle at which fluorescence intensity begins to increase exponentially, based on data regarding fluorescence intensity obtained by performing PCR on a target nucleic acid;
- a parameter analyzing unit which analyzes parameters related to amplification efficiency and nucleic acid concentration by curve-fitting a result of performing PCR on a reference nucleic acid with a known initial nucleic acid concentration;
- and
- a concentration estimating unit which estimates the initial nucleic acid concentration of the target nucleic acid by performing curve-fitting on the determined curve-fitting area by using the analyzed parameters.
Dependencies are inferred from the claim text.