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US 2014/0005439 A1Published application
Methods and Compositions for the Recombinant Biosynthesis of N-Alkanes
AssigneeJoule Unlimited Technologies, Inc.
InventorsNikos Basil Reppas, Christian Perry Ridley
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Abstract
The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into n-alkanes, and in particular the use of such organisms for the commercial production of n-alkanes and related molecules.
Claims14
A method for producing hydrocarbons, comprising:
- (i) culturing an engineered cyanobacterium in a culture medium, wherein said engineered cyanobacterium comprises a recombinant AAR enzyme and a recombinant ADM enzyme;
- and
- (ii) exposing said engineered cyanobacterium to light and carbon dioxide, wherein said exposure results in the conversion of said carbon dioxide by said engineered cynanobacterium into n-alkanes, wherein at least one of said n-alkanes is selected from the group consisting of n-tridecane, n-tetradecane, n-pentadecane, n-hexadecane, and n-heptadecane, and wherein the amount of said n-alkanes produced is between 0.1% and 5% dry cell weight and at least two times the amount produced by an otherwise identical cyanobacterium, cultured under identical conditions, but lacking said recombinant AAR and ADM enzymes.
An engineered cyanobacterium, wherein said engineered cyanobacterium comprises a recombinant AAR enzyme and a recombinant ADM enzyme.
Dependencies are inferred from the claim text.