Heteroarylcyanine Dyes

A fluorescent compound having the formula:

• A-Q-B
• wherein
• Q has a formula which is a member selected from:
• wherein
• D is a member selected from H, halogen, ORa, SRa, NRaRb, CRaRbRc,
• wherein
• Ra, Rb, Rc and Rd are members independently selected from H, R8, R8Zo, R8CO2H, R8SO3H,
• wherein
• Zo and Rq are independently selected from OH, O—, a reactive functional group, a component of a reactive functional group and a linkage fragment covalently binding said compound to a carrier molecule;
• v is 0, 1, 2, 3, 4, or 5 and, when v is equal to or greater than 2, each Rd moiety is independently selected;
• e is 0, 1, 2, 3, 4, or 5 and, when e is equal to or greater than 2, each Rd or Rq is independently selected;
• Re is selected from H, R18, R18Z1, SO3H, CO2H, R18SO3H, CONHR18SO3H, CONHR18C(O)Z1,
• wherein
• R8 and R18 are independently selected from a bond, substituted or unsubstituted substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl;
• Z1 is selected from OH, O—, a reactive functional group, a component of a reactive functional group and a linkage fragment covalently binding said compound to a carrier molecule;
• n is 1, 2 or 3;
• p is 0, 1 or 2;
• and
• Rm is a member selected from:
• wherein
• w is an integer from 1 to 10;
• u is 0, 1, 2, 3, 4, or 5 and, when u is two or greater, each Ro is independently selected;
• Ro is a member selected from OH, O—, a reactive functional group, a component of a reactive functional group and a linkage fragment binding the compound to a carrier molecule;
• B is a moiety which is a member selected from Formulae I, II and III;
• wherein
• X1 is a member selected from CR1R2, O, S and NR1,
• wherein
• R1, R2 and R3 are independently CH2R7;
• X2 and X3 are members independently selected from O, S, N, NH, CR22, and CR22R23,
• wherein
• R4, R5, R6, R7, R22 and R23 are members independently selected from H, R28, R28Z2, SO3H, CO2H, R28SO3H, C(O)NHR28SO3H, CONHR28C(O)Z2,
• wherein
• Rp and Z2 are independently selected from OH, O—, a reactive functional group, a component of a reactive functional group and a linkage fragment covalently binding said compound to a carrier molecule;
• g is an integer selected from 0, 1, 2, 3, and 4, and when g is greater than or equal to 2, each Rg or Rp is independently selected;
• Rg is a member selected from H, R38, R38Z3, SO3H, CO2H, R38SO3H, CONHR38SO3H, CONHR38C(O)Z2,
• wherein
• R28 and R38 are independently selected from a bond substituted or unsubstituted substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl;
• Z3 is a reactive functional group or is a linkage fragment covalently binding said compound to a carrier molecule;
• A is a moiety selected from substituted or unsubstituted indole, substituted or unsubstituted imidazole, substituted or unsubstituted thiazole, and substituted or unsubstituted oxazole,
• at least one of Ra, Rb, Rc, Rd, R1, R2, R3, R4, R5 and R6 is a reactive functional group, a component of a reactive functional group or is a linkage fragment covalently binding said compound to a carrier molecule;
• and
• said compound comprises at least three SO3H moieties.