Method for in vivo residue-specific DOPA incorporation into mussel adhesive proteins
AssigneePostech Academy-Industry Foundation
InventorsHyung Joon CHA, Byeongseon Yang, Yoo Seong Choi, Hyungdon Yun
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Abstract
The present invention relates to recombinant mussel adhesive protein wherein a DOPA residue is in vivo incorporated instead of a tyrosine residue, and a method for producing the same. More specifically, the present invention relates to recombinant mussel adhesive protein wherein a DOPA residue is incorporated instead of a tyrosine residue, and a method for producing the same, and a transformant for producing the recombinant mussel adhesive protein.
Claims20
A method for producing mussel adhesive protein wherein a tyrosine residue is substituted by DOPA or DOPA o-quinone, comprising the steps of:
- (1) introducing a vector that comprises nucleic acid encoding mussel adhesive protein in tyrosine auxotroph to prepare a transformant;
- (2) culturing the prepared transformant in a tyrosine-free medium to a stationary phase;
- and
- (3) when the cells reach a stationary phase, adding DOPA (3,4-dihydroxyphenylalanine) or DOPA o-quinone to the medium, and additionally culturing.
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A transformant prepared by transforming tyrosine auxotroph with a vector that comprises nucleic acid encoding mussel adhesive protein.
Dependencies are inferred from the claim text.