Pectate Lyase Variants

A variant of a parent enzyme having pectate lyase activity (EC 4.2.2.2) and comprising an alteration at one or more positions selected from the group consisting of positions number:

• 5, 9, 11, 26, 28, 30, 31, 37, 40, 45, 46, 47, 48, 49, 50, 51, 52, 54, 61, 64, 68, 69, 70, 71, 74, 75, 76, 79, 86, 87, 91, 99, 105, 106, 107, 111, 115, 116, 118, 122, 123, 134, 136, 139, 140, 141, 146, 148, 156, 158, 170, 182, 185, 186, 189, 193, 194, 196, 199, 201, 202, 204, 213, 215, 218, 224, 228, 229, 234, 235, 237, 251, 256, 257, 258, 272, 277, 286, 295, 298, 301, 302, 303, 305, 307, 308, 314, 316, 323, 324, 326, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 349, 356, 357, 363, 366, 378, 381, 384, 386, 387, 389, 390, 391, 393 and 397, wherein the alteration(s) are independently
• (i) an insertion of an amino acid downstream of the amino acid which occupies the position,
• (ii) a deletion of the amino acid which occupies the position, or
• (iii) a substitution of the amino acid which occupies the position with a different amino acid, and
• wherein each position corresponds to a position of the amino acid sequence of the pectate lyase having the amino acid sequence of SEQ ID NO:
• 2, and wherein the parent enzyme is the pectate lyase shown on SEQ ID NO:
• 2 or a pectate lyase having at least 65% identity to the amino acid sequence of SEQ ID NO:
• 2.