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US 2014/0005076 A1Published application
Methods for Identifying and Isolating Cells Expressing a Polypeptide
AssigneeOncoMed Pharmaceuticals, Inc.
InventorsAustin L. GURNEY, Alexandra L.L. Lazetic, Christopher J. Bond
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Abstract
The invention relates to novel polypeptides and cells comprising the polypeptides. The polypeptides and cells are used in methods to identify and/or isolate cells producing a protein with specific biological functions. In particular, the methods may be used for identifying, selecting, and isolating cells producing antigen-specific monoclonal antibodies.
Claims26
1-216. (canceled)
A cell library, each cell comprising:
- (a) a first polypeptide comprising:
- (i) an extracellular portion comprising an immunoglobulin heavy chain constant region comprising CH2 and CH3 domains;
- and
- (ii) a non-immunoglobulin transmembrane portion;
- and
- (b) a second polypeptide comprising an immunoglobulin heavy chain constant region comprising CH2 and CH3 domains,
- wherein the first and second polypeptides form a heterodimeric molecule that is expressed on the surface of the cell.
4 more elements
A cell library expressing heterodimeric molecules on the cell surface, comprising a population of cells that express:
- (a) a first polypeptide comprising an extracellular portion comprising an immunoglobulin heavy chain constant region comprising CH2 and CH3 domains, and a non-immunoglobulin transmembrane portion;
- and
- (b) a second polypeptide comprises an immunoglobulin heavy chain constant region comprising CH2 and CH3 domains.
Dependencies are inferred from the claim text.