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US 2014/0005073 A1Published application
Method and apparatus for analyzing nucleic acid by compensating for crosstalk in polymerase chain reaction data and other data
AssigneeSamsung Electronics Co., Ltd.
InventorsKyung-ho KIM, Soo-kwan LEE, Joon-ho KIM, Kak NAMKOONG, Sun-ok JUNG, Won-seok CHUNG
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Abstract
A method of analyzing nucleic acid by compensating for crosstalk in polymerase chain reaction (PCR) data and other data, wherein crosstalk signals associated with multiple fluorescent dyes are corrected by using fluorescent intensity variations detected from a concentration difference of the fluorescent dyes, and apparatus for performing the method.
Claims20
A nucleic acid analysis method comprising:
- obtaining a difference in concentration between different fluorescent dyes labeled on a nucleic acid by using a difference between fluorescent intensity variations detected from the fluorescent dyes;
- correcting crosstalk signals due to the fluorescent dyes by using the obtained difference in concentration;
- and
- estimating final threshold cycles of the fluorescent dyes by compensating for the detected fluorescent intensity variations by using the corrected crosstalk signals.
1 more element
A nucleic acid analysis apparatus comprising:
- a concentration analysis unit to obtain a difference in concentration between different fluorescent dyes labeled on a nucleic acid by using a difference between fluorescent intensity variations detected from the fluorescent dyes;
- a correction unit to correct crosstalk signals due to the fluorescent dyes by using the obtained difference in concentration;
- and
- an estimation unit to estimate final threshold cycles of the fluorescent dyes by compensating for the detected fluorescent intensity variations by using the corrected crosstalk signals.
1 more element
Dependencies are inferred from the claim text.