Glycoprofiling with multiplexed suspension arrays
AssigneesGlycosensors and Diagnostics, LLC, University of Georgia Research Foundation, Inc.
InventorsLoretta Yang, Robert J. Woods
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Abstract
The present invention is includes compositions and methods directed to the multiplexed analysis of carbohydrates and carbohydrate containing compounds. The compositions and methods utilize suspension array technology (SAT) and an array of different carbohydrate binding molecules, each carbohydrate binding molecules with a known carbohydrate binding specificity, to obtain a glycoprofile of the carbohydrate structure(s) in a sample. Each carbohydrate binding molecule of a given specificity is linked to the external surface of a population of individually addressable particles.
Claims25
A composition comprising a plurality of individually addressable particles, each individually addressable particle comprising an external surface and having linked to said external surface a separate carbohydrate binding molecule.
(canceled)
13-14. (canceled)
A kit comprising one or more compositions, each composition comprising individually addressable particles;
- wherein each individually addressable particle comprises an external surface and having linked to said external surface a separate carbohydrate binding molecule;
- and
- wherein each individually addressable particle is separately labeled with a detectable label.
16-21. (canceled)
A multiplex detection method for detecting a carbohydrate or a carbohydrate containing compound in a sample comprising:
- contacting the sample with a solution comprising a plurality of individually addressable particles, each individually addressable particle comprising an external surface and having linked to said external surface a separate carbohydrate binding molecule;
- and
- detecting the binding of the carbohydrate or carbohydrate containing compound to one more individually addressable particles;
- wherein the carbohydrate or carbohydrate containing compound bound to one more individually addressable particles remains in suspension.
(canceled)
31-50. (canceled)
Dependencies are inferred from the claim text.