Protein detection method
AssigneeThe Governing Council of the University of Toronto
InventorsJagotamoy Das, Shana O. Kelley
Life of this patent
Estimating the term…
Abstract
Described herein are systems and methods for detecting a target analyte in a sample with electrodes, comprising a linker and an antibody attached to the linker, and measuring an electrocatalytic signal changes generated by binding of an analyte in the sample to the antibody. Also disclosed herein are kits for electrochemical detection of protein analytes.
Claims20
1-64. (canceled)
A detection system for electrochemically detecting a protein analyte, comprising:
- an electrode comprising a linker on its surface, wherein the linker is attached to an antibody or fragment thereof capable of binding a protein analyte;
- and
- a redox reporter.
A method for electrochemical detection of a protein analyte, comprising:
- contacting a first electrode comprising a linker on its surface, wherein the linker is attached to a first antibody or fragment thereof capable of binding a first protein analyte with a sample and a redox reporter;
- measuring a first electrochemical signal generated by the first antibody-labeled electrode and the redox reporter when a potential is applied;
- and
- comparing the first electrochemical signal to a signal of a control sample comprising no protein analyte;
- wherein a change of the first electrochemical signal detected relative to a first signal of a control sample comprising no protein analyte is indicative of the presence of the first protein analyte in the sample.
A method for monitoring progression or response in a subject having a cancer, comprising:
- providing a biological sample from the subject;
- contacting an electrode comprising a linker on its surface, wherein the linker is attached to an antibody or fragment thereof capable of binding a protein analyte, with the sample and a redox reporter, wherein the antibody or fragment thereof binds to a protein analyte;
- measuring an electrochemical signal generated by the antibody-labeled electrode and the redox reporter when a potential is applied;
- and
- comparing the electrochemical signal to a signal of a control sample comprising no protein analyte;
- wherein a change of the signal detected relative to a signal of a control sample comprising no protein analyte is indicative of the presence of the protein analyte in the sample.
Dependencies are inferred from the claim text.