Method and system for the analysis of high density cells samples
AssigneesBiodot, Inc., Accupath Diagnostic Laboratories, Inc. (D.B.A. U.S. Labs)
InventorsMathew Moore, Miriam Reyes, David Baunoch, Thomas C. Tisone, Brendan O'Farrell
Life of this patent
Estimating the term…
Abstract
Methods for forming cell arrays of multiple cell samples arranged substantially in a monolayer on a single substrate particularly suited for diagnostic analysis are disclosed. The cell arrays are formed with a high-speed dispensing apparatus capable of dispensing small volumes in precise, complex patterns. Also disclosed are substrates upon which cell arrays may be formed, and methods for conducting diagnostic analyses on the formed cell arrays.
Claims32
1-50. (canceled)
A method of forming a cell array comprising a plurality of discrete cell samples on a single substrate, comprising:
- aspirating a cell suspension from a source;
- providing relative motion between the cell suspension and the substrate;
- metering a predetermined amount of the cell suspension using a direct current fluid source;
- dispensing the metered amount of the cell suspension in the form of droplets onto the substrate so as to form a plurality of discrete cell samples, each discrete cell sample being arranged substantially in a monolayer on the substrate;
- and
- dispensing a predetermined amount of probe or stain to interact with at least one of the discrete cell samples.
A cell array fabrication system, comprising:
- a dispensing apparatus, comprising:
- a dispenser configured to eject one or more reagent droplets of a predetermined size onto a substrate;
- and
- a direct current fluid source hydraulically coupled to the dispenser and configured to meter a predetermined quantity of reagent to or into the dispenser;
- and
- a cell array comprising the substrate and a plurality of discrete cell samples formed thereon by operation of the dispensing apparatus;
- wherein the cells of each discrete cell sample are arranged substantially in a monolayer.
A substrate upon which a cell array is to be formed, comprising:
- a barcode region, a control region, and a test region;
- wherein the cell array comprises a plurality of discrete cell samples arranged substantially in a monolayer and wherein the barcode region comprises at least one of a slide tracking barcode, and a dispense and lock barcode.
Dependencies are inferred from the claim text.