Complex rna composition of bodily fluids
AssigneeInstitute for Systems Biology
InventorsDavid GALAS, Kai WANG, Paul WILMES
Life of this patent
Estimating the term…
Abstract
Determination of the level and identity of various RNA molecules in circulation or tissues of subjects permits determination of microbiome composition and function of the subject as well as providing an index to health of various organs. Because rapid parallel sequencing techniques are available, such determinations can be conducted on a practical level.
Claims19
A database contained on a computer readable medium which comprises a record of the identity and levels of RNA sequences or molecules contained in an RNA spectrum associated with at least one of:
- 1) tissue or biological fluid of normal subjects;
- 2) tissue or biological fluid of subjects affected by known diseases or conditions;
- 3) tissue or biological fluid of subjects or administered known treatments;
- 4) tissue or biological fluid of subjects known to have ingested specified substances.
A method to determine a microbiome of a test subject, which method comprises the steps of
- a) obtaining a test RNA spectrum of RNA sequences or molecules in a sample of a tissue or biological fluid from said test subject;
- and
- b) associating the identity and/or level of RNA molecules in said spectrum with individual microorganisms and/or their function;
- whereby the microbiome of said subject is determined.
A method to determine whether a test subject has ingested one or more substances which method comprises the steps of
- a) obtaining a test RNA spectrum of RNA sequences or molecules in a sample of a tissue or biological fluid from said subject;
- and
- b) associating the identity and/or level of RNA molecules in said spectrum with said one or more substances;
- whereby assessing the presence and/or level of one or more of said RNA molecules as characteristic of said one or more substances determines whether said ingestion has occurred.
A method to expose circulating RNA molecules in the blood, plasma or serum which method comprises treating said blood, plasma or serum with an effective amount of protease and/or lipase.
A method to identify a biological pathway that is affected in a subject afflicted with an abnormal condition, which method comprises
- identifying at least one RNA molecule in the RNA spectrum of a sample of tissue or biological fluid of said subject, the presence or level of which is different in from that in a control spectrum comparably obtained from control subjects;
- testing the effect of said RNA molecule on the transcriptome of cells of the same species as the test subject;
- identifying at least one element of said transcriptome that is affected;
- and
- associating said element with a biological pathway.
Dependencies are inferred from the claim text.