Specific analysis of ketone and aldehyde analytes using reagent compounds, labeling strategies, and mass spectrometry workflow
AssigneeDH Technologies Development Pte. Ltd.
InventorsSubhakar Dey, Sasi Pillal, Brian L. Williamson, Subhasish Purkayastha, Michal Weinstock
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Abstract
Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.
Claims20
A set of mass labels comprising two or more compounds, wherein at least one of the two or more compounds is a compound of the formula:
- Y—(CH2)n-ONH2
- wherein n is an integer from 1 to 100 and Y is selected from the group consisting of:
A method for mass analysis of an analyte in a sample comprising:
- derivatizing an analyte comprising an aldehyde or ketone functional group, with a labeling reagent of formula:
- Y—(CH2)n-ONH2
- wherein n is an integer from 1 to 100 and Y is a group containing a net positive charge, to form a labeled analyte;
- subjecting the labeled analyte to ionization;
- and
- detecting the analyte by mass analysis.
Dependencies are inferred from the claim text.