Targeted alteration of dna
AssigneeKeygene N.V.
InventorsPaul Bundock, Franck Lhuissier
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Abstract
The current invention relates to a method for targeted alteration of acceptor DNA, for example duplex acceptor DNA. The method comprises the use of an oligonucleotide having at least one mismatch relative to the targeted (duplex) acceptor DNA. The mismatch is located at specific positions within said oligonucleotide. Also provided is a kit that comprises instructions for performing the method according to the inventions, and in a preferred embodiment, comprises an oligonucleotide suitable for use in the method.
Claims23
A method for targeted alteration of a duplex acceptor DNA sequence comprising the step of combining the duplex acceptor DNA sequence with a donor oligonucleotide, wherein the duplex acceptor DNA sequence comprises a first DNA sequence and a second DNA sequence, which is the complement of the first DNA sequence, and wherein the donor oligonucleotide comprises at least one domain that is capable of hybridizing to the first DNA sequence, and wherein the domain comprises or is directly adjacent to at least one mismatch with respect to the first DNA sequence, and wherein said at least one mismatch is positioned at most 2 nucleotides from the 3′ end of said donor oligonucleotide.
-17. (canceled)
Dependencies are inferred from the claim text.