Synthetic pathways for biofuel synthesis
AssigneeThe Regents of the University of California
InventorsMichelle C.Y. CHANG, Brooks Bond-Watts, Miao Wen, Jeffrey A. Hanson
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Abstract
The present disclosure provides optimized recombinant cells for the production of n-butanol. Methods for the use of these cells are also provided. Specifically, the utility of acylating aldehyde dehydrogenases and pyruvate:flavodoxin/ferredoxin-oxidoreductase for the improvement of n-butanol yields from recombinant cells is disclosed.
Claims39
A recombinant cell for the synthesis of n-butanol, the cell comprising:
- i. a recombinant sequence encoding an acylating aldehyde dehydrogenase catalyzing the conversion of acetaldehyde to acetyl-CoA,
- ii. a recombinant sequence encoding a keto-thiolase or acetyl-CoA acetyltransferase catalyzing the conversion of acetyl-CoA to acetoacetyl-CoA,
- iii. a recombinant sequence encoding an acetoacetyl-CoA reductase or hydroxybutyryl-CoA dehydrogenase catalyzing the conversion of acetoacetyl-CoA to 3-hydroxybutyryl-CoA,
- iv. a recombinant sequence encoding a crotonase catalyzing the conversion of 3-hydroxybutyryl-CoA to crotonyl-CoA,
- v. a recombinant sequence encoding a crotonyl-CoA reductase, butyryl-CoA dehydrogenase or trans-enoyl-CoA reductase catalyzing the conversion of crotonyl-CoA to butyryl-CoA, and
- vi. a recombinant sequence encoding a butyraldehyde/butanol dehydrogenase catalyzing the conversion of butyryl-CoA to n-butanol.
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