Immobilized interfacial enzymes of improved and stabilized activity
AssigneeTransbiodiesel Ltd.
InventorSobhi BASHEER
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Abstract
Disclosed is a process for the preparation of an interfacial enzyme immobilized on an insoluble support, by providing a bi-phase system comprised of an aqueous buffer solution and at least one first organic solvent; mixing said interfacial enzyme with the bi-phase system provided; adding the support to the obtained mixture and mixing; and isolating from the mixture obtained in the last step the interfacial enzyme immobilized on said support. The produced enzyme is locked in its catalytically active confirmation, and thus exhibits improved activity and stability. Also disclosed are uses of the produced enzymes, particularly in the preparation of biodiesel.
Claims20
A process for the preparation of an interfacial enzyme immobilized on an insoluble support, comprising the steps of:
- (a) providing a bi-phase system comprised of an aqueous buffer solution and at least one first organic solvent;
- (b) mixing said interfacial enzyme with the bi-phase system provided in step (a);
- (c) adding said support to the mixture of step (b) and mixing;
- (d) isolating from the mixture obtained in step (c) the interfacial enzyme immobilized on said support.
The process of any one of claims 1 to 8, wherein said enzyme is a lipase or a phospholipase.
An interfacial enzyme immobilized on a solid support, wherein said enzyme is locked in its catalytically active confirmation.
An interfacial enzyme immobilized on a solid porous support, wherein said support is homogenously covered with a surfactant, preferably with a monolayer of said surfactant.
Dependencies are inferred from the claim text.