US 2014/0004547 A1 · Rapid Tests for the Detection of Inhibitors of Enzymes and Human Exposure to the Same

Abstract

A device and method for the rapid on-site detection of inhibitors of enzymes, such as, acetylcholinesterase is described where the device contains 2 reaction zones containing a reporter enzyme substrate. One reaction zone is for the test sample while the other is for an onboard negative control. Sample and control fluids are preincubated with the enzyme in separate reaction containers, then an aliquot of each reaction mixture is added to designated reaction zones on the test device. A purpose-built reader or an illuminating device, such as, containing an incandescent light source, a diode, a UV light source or any other illumination source that is suitable for the reporter or mere visualization is used to determine the level of reporter.

Claims20

Dependent claims

A test system for detecting enzyme inhibitors comprising:

a) a device comprising two reaction zones, each reaction zone comprising a porous matrix comprising an immobilized reporter substrate of said enzyme, wherein said substrate is a reporter for activity of said enzyme;
and
b) separate from the device, two reaction containers comprising said enzyme, and optionally,
c) separate from the device, a reader that detects said reporter substrate.

1 more element

Dependencies are inferred from the claim text.

Rapid Tests for the Detection of Inhibitors of Enzymes and Human Exposure to the Same

Life of this patent

Rapid Tests for the Detection of Inhibitors of Enzymes and Human Exposure to the Same

Life of this patent

Abstract

Claims20