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US 2014/0004539 A1Published application
Systems and methods for multiplex solution assays
AssigneeThe Regents of the University of Michigan
InventorsArlyne Simon, John Frampton, Josh White, Shuichi Takayama
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Abstract
The present invention relates to systems and methods for an aqueous 2-phase system droplet array and uses thereof. The present invention further relates to methods for performing multiplexed assays, chemical, and/or biological experiments and tests (e.g., immunoassays).
Claims20
An assay system, comprising:
- a) a solid support comprising at least one first defined area;
- and
- b) a first solution comprising a first polymer or solute and reagents for detecting the presence or absence of one or more analytes in a test sample;
- and
- c) a second solution comprising a second polymer or solute and said test sample, wherein said second solution has a different density than said first solution, and wherein said first and second solutions form an aqueous two-phase system when mixed.
2 more elements
A method, comprising:
- a) contacting a solid support comprising at least one first defined area;
- and two or more second defined areas within said first defined area;
- with:
- i) a first solution comprising a first polymer or solute and reagents for detecting the presence or absence of at least one analyte in a test sample;
- and ii) a second solution comprising a second polymer or solute and said test sample, wherein said second solution has a different density than said first solution, and wherein said first and second solutions form an aqueous two-phase system when mixed;
- and
- b) detecting the presence of said analyte in said sample.
4 more elements
Dependencies are inferred from the claim text.