Method for detecting cellular dna damage and antibody against cell surface antigen responsive to dna strand breaks
AssigneesPerseus Proteomics Inc., Fujita Health University
InventorsYoshikazu KUROSAWA, Maiko KUROSAWA, Gene KUROSAWA
Life of this patent
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Abstract
To provide a method for detecting cellular DNA damage by specifically recognizing molecules responsive to DNA strand breaks. A method for detecting DNA damage in cells, which comprises administering in vivo, to cells, an antibody against a cell membrane surface antigen that is expressed in cells undergoing DNA strand breaks to a greater extent than in cells not undergoing DNA strand breaks in an environment that causes DNA damage, and analyzing the expression status of the cell membrane surface antigen.
Claims23
A method for detecting DNA damage in cells, which comprises administering in vivo, to cells, an antibody against a cell membrane surface antigen that is expressed in cells undergoing DNA strand breaks to a greater extent than in cells not undergoing DNA strand breaks in an environment that causes DNA damagemm, and analyzing the expression status of the cell membrane surface antigen.
An antibody that specifically reacts with human Ly6D having the amino acid sequences of SEQ ID NOS:
- 3, 4, and 5 as a heavy chain first complementarity-determining region (VH CDR1), a heavy chain second complementarity-determining region (VH CDR2), and a heavy chain third complementarity-determining region (VH CDR3).
An antibody that specifically reacts with human Ly6D having:
- a heavy chain variable region comprising a heavy chain first complementarity-determining region of SEQ ID NO:
- 3 (VH CDR1), a heavy chain second complementarity-determining region of SEQ ID NO:
- 4 (VH CDR2), and a heavy chain third complementarity-determining region of SEQ ID NO:
- 5;
- and a light chain variable region comprising a light chain first complementarity-determining region of SEQ ID NO:
- 6 (VL CDR1), a light chain second complementarity-determining region of SEQ ID NO:
- 7 (VL CDR2), and a light chain third complementarity-determining region of SEQ ID NO:
- 8 (VL CDR).
Dependencies are inferred from the claim text.