Nucleic Acids Encoding a Mammalian Raptor Polypeptide and Uses Therefor
AssigneeWhitehead Institute for Biomedical Research
InventorsDavid M. Sabatini, Do-Hyung Kim, Dos Sarbassov
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Abstract
The present invention relates to isolated raptor nucleic acid molecules of mammalian origin (e.g., human) and complements, portions and variants thereof. Another aspect of the invention are isolated raptor polypeptides of mammalian origin and portions thereof, and antibodies or antigen binding fragments thereof that specifically bind a raptor polypeptide. The present invention also relates to constructs and host cells comprising the nucleic acid molecules described herein. In addition, the present invention relates to uses of the nucleic acid and polypeptide molecules provided herein.
Claims22
1-12. (canceled)
A method of identifying an agent that alters interaction of a mammalian raptor protein with mammalian target of rapamycin (mTOR) protein comprising:
- a) contacting a cell which comprises a nucleic acid molecule that encodes a raptor protein comprising an amino acid sequence at least 95% identical to SEQ ID NO:
- 2 wherein the raptor protein, when expressed, interacts with mTOR protein in the cell, with an agent to be assessed;
- b) assessing growth rate or size of the cell by a method comprising measuring phosphorylation of a regulator of protein synthesis, wherein the regulator of protein synthesis is optionally S6 kinase 1, 4E-BP1, or both, wherein altered phosphorylation of a regulator of protein synthesis indicates that the agent alters interaction of a mammalian raptor protein with mTOR protein.
17-24. (canceled)
An isolated complex comprising (i) a chimeric polypeptide comprising a polypeptide having an amino acid sequence at least 95% identical to SEQ ID NO:
- 2;
- and (ii) a mammalian mTOR polypeptide.
A composition comprising (a) an isolated complex comprising (i) a first polypeptide at least 95% identical to SEQ ID NO:
- 2;
- and (ii) a second polypeptide comprising a mammalian mTOR polypeptide;
- and (b) a buffer appropriate to preserve the interaction between the polypeptide of SEQ ID NO:
- 2 and mTOR.
Dependencies are inferred from the claim text.