Method for the evaluation of compound-target interactions across species
AssigneeCellzome Limited
InventorsMarcus Bantscheff, Mikhail Savitski
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Abstract
The present invention relates to a method for the determination of the species profile of a protein-interacting compound, comprising the steps of a) providing a protein preparation derived from one species (species-1) containing a species-1-variant of said protein, b) providing a protein preparation derived from another species (species-2) containing a species-2-variant of said protein, c) mixing said preparations, d) contacting said mixed protein preparations with a ligand of said protein and with a given protein-interacting compound under conditions allowing the formation of a complex between said ligand and said protein, and e) detecting the complexes formed in step d) with the help of mass spectrometry.
Claims14
A method for the determination of the species profile of a protein-interacting compound, comprising the steps of
- a) providing a protein preparation derived from one species (species-1) containing a species-1-variant of said protein,
- b) providing a protein preparation derived from another species (species-2) containing a species-2-variant of said protein,
- c) mixing said protein preparations,
- d) contacting said mixed protein preparations with a ligand of said protein and with a given protein-interacting compound under conditions allowing the formation of a complex between said ligand and said protein,
- e) detecting the complexes formed in step d) with the help of mass spectrometry.
Dependencies are inferred from the claim text.