Method for Detecting a Target Analyte Using a Self-Cleaving Synthetic Molecule
AssigneeQURA Scientific, LLC
InventorHeath Bowers
Life of this patent
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Abstract
One aspect of the present disclosure relates to a method for determining the amount of a target analyte in a sample. One step of the method can include contacting the sample with a self-cleaving synthetic molecule to form a first treated sample. The self-cleaving synthetic molecule can include at least one target analyte-specific aptamer. Next, the first treated sample can be contacted with at least one actuating molecule to form a second treated sample. The second treated sample is then subjected to a target amplification assay. A detectable signal is produced by the target amplification assay when the target analyte is present in the sample.
Claims20
A method for determining the amount of a target analyte in a sample, the method comprising (a) contacting the sample with a self-cleaving synthetic molecule to form a first treated sample, the self-cleaving synthetic molecule including at least one target analyte-specific aptamer, (b) contacting the first treated sample with at least one actuating molecule to form a second treated sample, and (c) subjecting the second treated sample to a target amplification assay, wherein a detectable signal is produced by the target amplification assay when the target analyte is present in the sample.
A method for determining the amount of a target polypeptide in a sample, the method comprising (a) contacting the sample with a type I hammerhead ribozyme to form a first treated sample, the type I hammerhead ribozyme including at least one target analyte-specific aptamer and first and second primer sites at the 3′ and 5′ ends thereof, respectively, (b) contacting the first treated sample with at least one actuating molecule to form a second treated sample, and (c) subjecting the second treated sample to RT-qPCR, wherein the 3′ primer site is not cleaved from the type I hammerhead ribozyme in the presence of the target analyte and wherein an uncleaved target analyte-specific aptamer is amplified by the target amplification assay to produce a detectable signal.
Dependencies are inferred from the claim text.