MicroRNA-Based Methods for Prognosis of Hepatocellular Carcinoma
OwnerChau-Ting Yeh, Ya-Hui Huang +2inventors
InventorsChau-Ting Yeh, Ya-Hui Huang, Hua-Chien Chen, Kwang-Huei Lin
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Abstract
Disclosed herein is a method for determining the prognosis of a subject diagnosed with hepatocellular carcinoma based on the expression level of at least one microRNA in the non-cancerous liver tissue of the subject. According to various embodiments of the present disclosure, expression levels of miR-486-3p, miR-876-5p, and miR-381 are positively associated with a favorable prognosis, while expression levels of miR-30c, miR-432, and miR-15b are negatively associated with a favorable prognosis.
Claims20
A method for determining the prognosis of a subject diagnosed with hepatocellular carcinoma, comprising,
- (a) obtaining a non-cancerous sample from the non-cancerous liver tissue of the subject;
- (b) detecting the expression level (Ctmir) of a microRNA in the non-cancerous sample, wherein the microRNA is at least one microRNA selected from a first group consisting of miR-486-3p, miR-876-5p, and miR-381, or a second group consisting of miR-30c, miR-432, and miR-15b;
- (c) comparing the Ctmir of the microRNA or a parameter derived therefrom with a cutoff value associated with the microRNA for classifying the microRNA into (1) a high-expression type when the Ctmir of the microRNA or the parameter derived therefrom is greater than the cutoff value associated with the microRNA, or (2) a low-expression type when the Ctmir of the microRNA or the parameter derived therefrom is smaller than the cutoff value associated with the microRNA, wherein
- the presence of a high-expression type microRNA from the first group indicates a favorable prognosis for the subject;
- while the presence of a low-expression type microRNA from the first group indicates an unfavorable prognosis for the subject;
- and
- the presence of a low-expression type microRNA from the second group indicates a favorable prognosis for the subject;
- while the presence of a high-expression type microRNA from the second group indicates an unfavorable prognosis for the subject.
A method for determining the prognosis of a subject diagnosed with hepatocellular carcinoma, comprising,
- (a) obtaining a non-cancerous sample from the non-cancerous liver tissue of the subject;
- (b) detecting the expression level (Ctmir) of miR-486-3p and the expression level (Ctctrl) of miR-30d in the non-cancerous sample;
- (c-1) calculating a normalized expression level (ΔCtmir) of the miR-486-3p according to equation (1):
- normalized expression level=Ctctrl−Ctmir equation (1);
- (c-2) calculating an amplified normalized expression level (amplified ΔCtmir) of the miR-486-3p according to equation (2):
- amplified expression level=2ΔCtmir×106 equation (2);
- and
- (c-3) comparing the amplified ΔCtmir of the miR-486-3p with a cutoff value of 64,800, wherein
- the amplified ΔCtmir of the miR-486-3p being higher than the cutoff value indicates a favorable prognosis for the subject, and
- the amplified ΔCtmir of the miR-486-3p being lower than the cutoff value indicates an unfavorable prognosis for the subject.
A method for determining the prognosis of a subject diagnosed with hepatocellular carcinoma, comprising,
- (a) obtaining a non-cancerous sample from the non-cancerous liver tissue of the subject;
- (b) detecting the expression level (Ctmir) of miR-432 and the expression level (Ctctrl) of miR-30d in the non-cancerous sample;
- (c-1) calculating a normalized expression level (ΔCtmir) of the miR-432 according to equation (1):
- normalized expression level=Ctctrl−Ctmir equation (1);
- (c-2) calculating an amplified normalized expression level (amplified ΔCtmir) of the miR-486-3p according to equation (2):
- amplified expression level=2ΔCtmir×106 equation (2);
- and
- (c-3) comparing the amplified ΔCtmir of the miR-432 with a cutoff value of 19,300, wherein
- the amplified ΔCtmir of the miR-432 being lower than the cutoff value indicates a favorable prognosis for the subject, and
- the amplified ΔCtmir of the miR-432 being higher than the cutoff value indicates an unfavorable prognosis for the subject.
Dependencies are inferred from the claim text.