Method for identifying polymorphism of nucleic acid molecules

A method for identifying polymorphism of nucleic acid molecules comprising:

• (a) preparing a sample solution comprising a first nucleic acid probe, which specifically hybridizes with a single-stranded nucleic acid molecule including a first type of base sequence in a polymorphic sequence, and a target nucleic acid molecule;
• (b) forming a hybrid of the nucleic acid molecules in the sample solution prepared in (a);
• (c) calculating a number of molecules of the hybrid including the first nucleic acid probe in the sample solution prepared in (a) after carrying out (b);
• (d) identifying polymorphism of the target nucleic acid molecule based on a result of (c);
• (e) moving a location of a photodetection region of an optics system in the sample solution in (c) using an optics system of a confocal microscope or a multiphoton microscope;
• (f) detecting fluorescence emitted from the hybrid in the photodetection region while moving the location of the photodetection region of the optics system in the sample solution in (c);
• (g) individually detecting the hybrid in (c) by individually detecting an optical signal from each hybrid among the detected fluorescent light;
• and
• (h) counting a number of particles detected during movement of the location of the photodetection region in (c) by counting the number of individually detected hybrid, wherein
• the sample solution in (a) or the sample solution after (b) and before (c) includes an oligonucleotide having a base sequence complementary to a base sequence different from the first type of base sequence in the polymorphic sequence.