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US 2014/0004511 A1Published application
Identification of 5-methyl-c in nucleic acid templates
AssigneesThe University of Chicago, Pacific Biosciences of California, Inc.
InventorsJonas Korlach, Chuan He, Tyson A. Clark, Liang Zhang, Xingyu Lu
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Abstract
A method for identifying a 5-MeC in a template nucleic is provided. The method comprises providing a template having 5-MeC, converting the 5-MeC into a futher modification selected from 5-caC and 5-FC. The converted template is then sequenced, and a change in sequencing is detected that is indicative of the further modification, allowing for identifying the 5-MeC in the template nucleic acid.
Claims17
A method for identifying a 5-MeC in a template nucleic acid, the method comprising:
- a) providing a template nucleic acid comprising the 5-MeC;
- b) converting the 5-MeC into a further modification by treating the template nucleic acid with a Tet protein, wherein the further modification is selected from 5-caC and 5-fC;
- c) sequencing the template nucleic acid;
- d) monitoring the sequencing;
- and
- e) detecting a change in the sequencing that is indicative of the further modification, thereby indentifying the 5-MeC in the template nucleic acid.
3 more elements
Dependencies are inferred from the claim text.