Kit for isothermal dna amplification starting from an rna template
AssigneeGeneral Electric Company
InventorsJohn Richard Nelson, Robert Scott Duthie, Gregory Andrew Grossmann, Ryan Charles Heller
Life of this patent
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Abstract
A kit for amplifying deoxynucleic acid (DNA) from ribonucleic acid (RNA) template is provided. The kit for amplifying a RNA comprises at least one inosine-containing primer; and at least one enzyme comprising a reverse transcriptase activity, a strand displacement DNA polymerase activity, a nuclease acitivity for nicking DNA 3′ to an inosine residue of the primer or combinations thereof. The kit further comprises one or more quantifying reagents to detect the presence of RNA in a sample or quantify the RNA present in a sample.
Claims29
A kit for amplifying a deoxyribonucleic acid (DNA) from a ribonucleic acid (RNA) template, comprising:
- at least one inosine-containing primer;
- and
- at least one reverse transcriptase, at least one strand displacement DNA polymerase, and at least one nuclease for nicking DNA 3′ to an inosine residue of the primer.
(canceled)
A kit for amplifying deoxyribonucleic acids (DNA) from a ribonucleic acid (RNA) template, comprising:
- at least one inosine-containing primer;
- an enzyme comprising reverse transcriptase activity and strand displacement DNA polymerase activity, and
- a nuclease for nicking DNA 3′ to an inosine residue of the primer.
A kit for amplifying deoxyribonucleic acids (DNA) from a ribonucleic acid (RNA) template, comprising:
- at least one inosine-containing primer;
- a reverse transcriptase;
- a strand displacement DNA polymerase comprising exonuclease deficient DNA polymerase activity;
- and
- a nuclease for nicking DNA 3′ to an ino sine residue of the primer.
(canceled)
A kit of quantifying a ribonucleic acid (RNA) template from a sample comprising:
- at least one inosine-containing primer,
- a reverse transcriptase, a strand displacement DNA polymerase;
- a nuclease for nicking DNA 3′ to an inosine residue of the primer;
- and
- a nucleic acid detection system.
Dependencies are inferred from the claim text.