Membrane-Based Lateral Flow Assay Devices that Utilize Phosphorescent Detection
AssigneeKimberly-Clark Worldwide, Inc.
InventorXuedong Song
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Abstract
A lateral flow, membrane-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes phosphorescence to detect the signals generated by excited phosphorescent labels. The labels may have a long emission lifetime so that background interference from many sources, such as scattered light and autofluorescence, is practically eliminated during detection. In addition, the phosphorescent labels may be encapsulated within particles to shield the labels from quenchers, such as oxygen or water, which might disrupt the phosphorescent signal.
Claims11
A method for detecting an analyte within a test sample, the method comprising:
- i) providing a lateral flow assay device that comprises a porous membrane in fluid communication with a phosphorescent particle, the phosphorescent particle comprising a phosphorescent label encapsulated within a barrier matrix comprising polymethylmethacrylate, the phosphorescent label capable of emitting a detection signal following excitation of the phosphorescent label, wherein the porous membrane defines a detection zone within which is immobilized a capture reagent;
- ii) contacting the lateral flow assay device with the test sample;
- iii) subjecting the detection zone to illumination pulses to generate the detection signal;
- and
- iv) thereafter, measuring the intensity of the detection signal, wherein the amount of the analyte within the test sample is proportional to the intensity of the detection signal.
11-16. (canceled)
Dependencies are inferred from the claim text.