Compositions and methods for the detection and analysis of african swine fever virus
AssigneeBrandeis University
InventorsLawrence J. Wangh, Bonnie Ronish
Life of this patent
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Abstract
Provided herein are compositions and methods for the detection and analysis of African swine fever virus (ASFV). In particular, kits, compositions, and methods employing LATE-PCR reagents and processes for the detection and analysis of ASFV are provided.
Claims21
-72. (canceled)
A method for detecting or analyzing African swine fever virus (ASFV) in a sample, comprising:
- contacting one or more copies of viral genomic DNA isolated, or purified, or separated, or prepared from said sample with reagents for performing LATE-PCR, said reagents comprising a limiting primer and an excess primer pair for at least one target sequence, wherein the initial concentration-dependent melting temperature of said limiting primer is equal to or greater than the initial concentration-dependent melting temperature of the paired excess primer for corresponding ASFV target sequence;
- and amplifying said target sequence to generate specific double-stranded and single-stranded DNA products.
A kit for detecting or analyzing African swine fever virus (ASFV) in a sample, comprising a limiting primer and an excess primer for LATE-PCR amplification of a target sequence of ASVF nucleic acid, wherein the initial concentration-dependent melting temperature of said limiting primer is equal to or greater than the initial concentration-dependent melting temperature of the corresponding excess primer for the ASFV target sequence.
Dependencies are inferred from the claim text.