US 2014/0004248 A1 · Processes of Purifying Steviol Glycosides

Abstract

A process for producing the natural sweetener composition which comprises at least one of steviolbioside (STB) extract, Rebaudioside B extract and Rebaudioside D extract (“collectively, the “extracts”) comprises the steps of preparing a mother liquor comprising a mass content of at least 20% of at least one of the extracts; preparing feed liquid comprising at least 20 mg/mL of mother liquor; flowing feed liquid through a porous adsorption column, having a pore size of at least 40 Angstroms, a pore volume of a least 0.8 mL/g and at a flow rate of at least 1L/min and at a pH of between 4 to 5; eluting at least one steviolbioside (STB) extract, Rebaudioside B extract and Rebaudioside D extract with alcohol having a mass concentration of at least 65%; fractionally collecting one or more eluates based on chromatographic critical points for each of the steviolbioside STB extract, the Rebaudioside B extract and the Rebaudioside D extract; concentrating the extracts at a temperature of between 60-80° C.; and drying the extracts so formed.

Claims30

Dependent claims

A process for producing the natural sweetener composition comprising at least one of steviolbioside (STB) extract, Rebaudioside B extract and Rebaudioside D extract (“collectively, the “extracts”), said process comprising the steps of:

a) preparing a mother liquor comprising a mass content of at least 20% of at least one of the extracts;
b) prepare feed liquid comprising at least 20 mg/mL of mother liquor;
c) flow feed liquid through a porous adsorption column, having a pore size of at least 40 Angstroms, a pore volume of a least 0.8 mL/g and at a flow rate of at least 1 L/min and at a pH of between 4 to 5;
d) eluting at least one steviolbioside (STB) extract, Rebaudioside B extract and Rebaudioside D extract with alcohol having a mass concentration of at least 65%;
e) fractionally collecting one or more eluates based on chromatographic critical points for each of the steviolbioside STB extract, the Rebaudioside B extract and the Rebaudioside D extract;
f) concentrating the extracts at a temperature of between 60-80° C.;
and
g) drying the extracts so formed.

5 more elements

A process for purifying rebaudioside B, which comprises:

preparing a mother liquor of into a feed liquid of 20-25 mg/ml;
allowing the feed liquid to flow through a macroporous adsorption resin column at a rate of 2.0-3.0 L/min with the average pore size of said macroporous adsorption resin column being from 50-60 A, with the pore volume thereof being 0.8-0.9 ml/g, and the pH thereof during the adsorption being in the range of 4.0-5.0;
and after substantially complete adsorption, eluting the Stevia rebaudiana glycoside adsorbed on the resin column by using ethanol with a mass concentration of 70%-75%, collecting fractionally the eluates, concentrating the eluates at a temperature of 60° C.-80° C., and separately drying the resulting solid and liquid to give a crude rebaudioside B Stevia rebaudiana glycoside.

A process for purifying rebaudioside D, characterized by preparing a mother liquor of sugar into a feed liquid of 25-30 mg/ml, allowing the feed liquid to flow through a macroporous adsorption resin column at a rate of 2.5-4.0 L/min, wherein the average pore size of said resin column is 40-50 A, with a pore volume thereof being 0.9-1.0 ml/g, and the pH thereof during the adsorption being in the range of 4.5-5.5;

and after substantially complete adsorption, eluting the Stevia rebaudiana glycoside adsorbed on the resin column by using ethanol with a mass concentration of 75%-80%;
collecting fractionally the eluates, determining the critical points, then collecting the eluates at the critical points, concentrating at a temperature of 60° C.-80° C., and separately drying the resulting solid and liquid to give a crude Stevia rebaudiana glycoside.

A process for purifying steviolbioside (STB), characterized by preparing a mother liquor of sugar into a feed liquid of 25-30 mg/mL, allowing the feed liquid to flow through a macroporous adsorption resin column in a rate of 1.0-2.0 L/min, wherein the average pore size of said resin column is 40-50 A, with a pore volume thereof being 0.9-1.0 mL/g, and the pH thereof during the adsorption being in the range of 4.0-5.0;

and after substantially complete adsorption, eluting the Stevia rebaudiana glycosides adsorbed on the resin column by using ethanol with a mass concentration of 67%-72%;
collecting fractionally the eluates, determining the critical points, then collecting the eluates at the critical points, concentrating at a temperature of 60° C.-80° C., and separately drying the resulting solid and liquid to give a crude Stevia rebaudiana glycoside.

Dependencies are inferred from the claim text.

Processes of Purifying Steviol Glycosides

Life of this patent

Processes of Purifying Steviol Glycosides

Life of this patent

Abstract

Claims30