Cell attachment coatings and methods using phosphorous-containing photoreagent
AssigneeSurModics, Inc.
InventorJoseph S. McGonigle
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Abstract
Cell attachment coatings for articles such as implantable medical devices and cell culture vessels are disclosed. The coatings include an intermediate coater layer which includes a phosphorous-containing component that is bonded in the coating by reacted photoreactive functional groups. The coating also include a second coated layer including an immobilized ECM protein or peptide that includes an active portion of an ECM protein that is able to serve as an outer layer to contact cells during use. The coatings promoted enhanced cell binding and growth.
Claims20
An article comprising a biocompatible cell attachment coating comprising:
- (a) an intermediate coated layer comprising a first component comprising (i) a phosphorous-containing group, (ii) a bonding group comprising a photoreactive group;
- wherein the first component is immobilized in the coating via the bonding group;
- and
- (b) a second coated layer comprising a cell attachment molecule comprising an extracellular matrix (ECM) protein, or a peptide that includes an active portion of an ECM protein that is immobilized in the coating;
- wherein the intermediate coated layer is positioned between the second coated layer and a surface of the article.
A method for forming a coated article comprising steps of:
- (a) applying a first composition on an article surface, the first composition comprising first component comprising (i) a phosphorous-containing group, (ii) a bonding group comprising photoreactive group;
- (b) providing a second composition on the article surface, the second composition comprising a cell attachment molecule comprising an ECM protein, or a peptide that includes an active portion of an ECM protein for cell attachment;
- (c) irradiating the device surface to cause bonding of the aryl functional group to a target moiety, wherein irradiating is performed after step (a), after step (b), or both after steps (a) and (b).
Dependencies are inferred from the claim text.