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US 2013/0330759 A1Published application
Enhancing endotoxin detection
AssigneeBioDtech, Inc.
InventorsMichael G. Pepe, Milton Keith Champion
Life of this patent
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Abstract
Provided herein are methods for detecting endotoxin or Gram negative bacteria in a sample. Kits for detecting endotoxin or Gram negative bacteria in a sample are provided.
Claims15
A method of detecting endotoxin in a sample comprising:
- a) heating the sample;
- b) acidifying the heated sample to a pH of about 1 to 4;
- c) contacting the acidified sample with an acidic protease;
- d) increasing the pH of the protease-treated sample to about 6 to 8 and
- e) detecting endotoxin in the sample.
2 more elements
A kit for detecting endotoxin in a sample comprising:
- a) an acidic protease;
- b) an acidic protease buffer;
- c) an acid;
- and
- d) a base
2 more elements
Dependencies are inferred from the claim text.