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AssigneeMascoma Corporation
InventorsWilliam Ryan Sillers, Shital A. Tripathi, Arthur J. Shaw, Aaron Argyros, David A. Hogsett
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The present invention provides for novel metabolic pathways to convert biomass and other carbohydrate sources to malonyl-CoA derived products, such as hydrocarbons and other bioproducts, under anaerobic conditions and with the net production of ATP. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to achieve conversion of a carbohydrate source to, e.g., long-chain hydrocarbons and hydrocarbon derivatives, wherein the one or more native and/or heterologous enzymes is activated, upregulated, downregulated, or deleted. The invention also provides for processes to convert biomass to malonyl-CoA derived products which comprise contacting a carbohydrate source with a recombinant microorganism of the invention.
A recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source to a hydrocarbon, wherein said one or more native and/or heterologous enzymes is activated, upregulated, downregulated, or deleted.
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A recombinant microorganism comprising an engineered metabolic pathway to direct metabolic flux from phosphoenolpyruvate to oxaloacetate rather than from phosphoenolpyruvate to pyruvate.
A method for identifying a transcarboxylase capable of converting oxaloacetate and acetyl-CoA to pyruvate and malonyl-CoA comprising:
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A recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source to a malonyl-CoA derived product, wherein said one or more native and/or heterologous enzymes is activated, upregulated, downregulated, or deleted.
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An isolated bacterial strain E. coli ΔaccC::matBC.
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Dependencies are inferred from the claim text.