Engineering microbes and metabolic pathways for the production of ethylene glycol
AssigneeMassachusetts Institute of Technology
InventorsGregory Stephanopoulos, Brian Pereira, Marjan De Mey, Deepak Dugar, Jose Luis Avalos
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Abstract
The invention relates to recombinant cells and their use in the production of ethylene glycol.
Claims53
A cell engineered to produce ethylene glycol, optionally wherein the cell has reduced or eliminated activity or reduced or eliminated expression of aldehyde dehydrogenase relative to a wild type cell, optionally wherein the aldehyde dehydrogenase is aldehyde dehydrogenase A and optionally wherein the cell is engineered to produce ethylene glycol from sugar, a pentose, xylose, L-arabinose, glucose, glycerol, serine, or a metabolite comprising glycolaldehyde.
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A method for producing ethylene glycol in a cell, comprising:
- reducing or eliminating the activity or expression of aldehyde dehydrogenase A and/or xylulokinase in the cell, relative to a wild type cell;
- increasing the expression of an enzyme that interconverts D-xylulose and D-ribulose, a D-ribulokinase, D-ribulose-phosphate aldolase, glycolaldehyde reductase and/or subunit of the E1 component of 2-oxoglutarate dehydrogenase in the cell, relative to a wild type cell;
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- culturing the cell.
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A method for producing ethylene glycol in a cell, comprising:
- reducing or eliminating the activity or expression of aldehyde dehydrogenase A and/or L-ribulokinase in the cell, relative to a wild type cell;
- increasing the expression of an enzyme that interconverts L-ribulose and L-xylulose, an ATP:L-xylulose 1-phosphotransferase, L-xylulose-1-phosphate aldolase, and glycolaldehyde reductase in the cell, relative to a wild type cell;
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- culturing the cell.
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Dependencies are inferred from the claim text.