Bioreactor with feed and harvest flow through filter assembly
AssigneeGe Health Care Bio-Sciences AB
InventorPatric Fricking
Life of this patent
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Abstract
The present invention relates to a bioreactor and a method for operating a bioreactor which comprises a chamber (100) capable of receiving a liquid media, and a filter assembly (200) comprising a perfusion filter (210; 212; 214). The filter assembly (200) is disposed in the chamber (100) and the filter assembly (200) is free to move within the chamber (100). The filter assembly (200) comprises means for coupling a harvesting flow through said filter (210; 212; 214) and means for coupling a feed flow through said filter in an opposite direction compared to the harvesting flow. Through this arrangement, the clogging prevention of the perfusion filter can be improved.
Claims10
A bioreactor comprising a chamber (100) capable of receiving a liquid media, and a filter assembly (200) comprising a perfusion filter (210;
- 212;
- 214), wherein the filter assembly (200) is disposed in the chamber (100) and the filter assembly (200) is free to move within the chamber (100), wherein the filter assembly (200) comprises means for coupling a harvesting flow through said filter (210;
- 212;
- 214) and means for coupling a feed flow through said filter in an opposite direction compared to the harvesting flow, wherein the feed flow is pumped to the bioreactor by means of a feed pump (330) and the harvest flow is pumped from the bioreactor by means of a harvest pump (340).
A method of operating a bioreactor comprising a chamber (100) capable of receiving a liquid media, and a filter assembly (200) comprising a perfusion filter (210;
- 212;
- 214), wherein the filter assembly (200) is disposed in the chamber (100) and the filter assembly (200) is free to move within the chamber (100), wherein the method comprises:
- removing a harvesting flow through said perfusion filter (210;
- 212;
- 214) from the bioreactor by means of a harvest pump (340);
- feeding a feed flow through said perfusion filter (210;
- 212;
- 214) to the bioreactor by means of a feed pump (330);
- wherein the harvesting flow is removed through said perfusion filter (210;
- 212;
- 214) in an opposite direction compared to the feed flow.
Dependencies are inferred from the claim text.