Microorganisms and processes for the production of isoprene
AssigneeGlycos Biotechnologies, Inc.
InventorsPaul Campbell, Sebastian Bredown, Huaijin Zhou, Stephanie Doneske, Daniel J. Monticello
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Abstract
The present invention provides a novel biosynthetic pathway for the production of isoprene from 3-methyl-2-buten-1-ol or 2-methyl-3-buten-2-ol. Further embodiments provide non-naturally occurring microorganism that have been modified to produce isoprene from 3-methyl-2-buten-1-ol or 2-methyl-3-buten-2-ol and methods of producing isoprene using said microorganism.
Claims20
A non-naturally occurring microbial organism comprising an isoprene biosynthetic pathway for the conversion of dimethylallyl diphosphate to isoprene, wherein the isoprene biosynthetic pathway comprises an exogenous nucleic acid encoding at least one enzyme of an isoprene biosynthetic pathway selected from:
- 2-methyl-3-buten-2-ol synthase;
- 2-methyl-3-buten-2-ol dehydratase;
- and any combination thereof;
- and wherein the isoprene biosynthetic pathway is expressed at a sufficient level to produce isoprene.
Dependencies are inferred from the claim text.