Aureusidin-producing transgenic plants
AssigneeJ.R. Simplot Company
InventorsCaius M. ROMMENS, Roshani SHAKYA, Jingsong YE
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Abstract
Aurone, including aureusidin-6-O-glucoside, are known to have antioxidant properties. The compounds are produced in the flowers snapdragon (e.g., Antirrhinum majus) and have been suggested for potential medicinal use. The present methods use recombinant and genetic methods to produce aurone in plants and plant products. In particular, the present methods have resulted in the production of aureusidin-6-O-glucoside in the leaves of various plants.
Claims20
A method for modifying a plant, comprising overexpressing or expressing de novo at least one of (i) chalcone 4′-O-glucosyltransferase, and (ii) aureusidin synthase, in said plant.
A modified plant comprising in its genome one or more exogenous genetic cassettes selected from the group consisting of (i) a gene expression cassette for expressing chalcone 4′-O-glucosyltransferase, and (ii) a gene expression cassette for expressing aureusidin synthase.
A transformation vector comprising one or more genetic cassettes selected from the group consisting of (i) a gene expression cassette for expressing chalcone 4′-O-glucosyltransferase, and (ii) a gene expression cassette for expressing aureusidin synthase.
A method comprising:
- (A) stably integrating into the genome of at least one plant cell (i) an exogenous gene expression cassette for expressing chalcone 4′-O-glucosyltransferase and (ii) an exogenous gene expression cassette for expressing aureusidin synthase, and (B) proliferating the transformed plant cell in the presence of naringenin chalcone.
Dependencies are inferred from the claim text.