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US 2013/0296535 A1Published application
High-Throughput Immune Sequencing
AssigneePresident and Fellows of Harvard College
InventorsGeorge M. Church, Francois Vigneault, Uri Laserson, Ido Bachelet
Life of this patent
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Abstract
Methods and compositions for determining and/or monitoring the immune state of an individual are provided.
Claims27
1-35. (canceled)
A method for identifying a therapeutic antibody, comprising:
- (a) linking polynucleotide sequences encoding a VH and a VL isolated from a single cell of a plurality of cells from a single subject to form paired sequences;
- (b) sequencing polynucleotides amplified from the paired sequences;
- (c) determining the frequency of two or more paired VH and VL sequences;
- (d) determining a variance from a reference library of the paired sequences from (c);
- and
- (e) identifying a therapeutic antibody based on (c) and (d).
3 more elements
Dependencies are inferred from the claim text.